product summary
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company name :
HUABIO
product type :
antibody
product name :
Cathepsin S
catalog :
HA500422
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of Cathepsin S on different lysates with Rabbit anti-Cathepsin S antibody (HA500422) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate (10 µg/Lane)
Lane 2: THP-1 cell lysate (10 µg/Lane)
Lane 3: Hela cell lysate (10 µg/Lane)
Lane 4: A431 cell lysate (10 µg/Lane)
Lane 5: PC-12 cell lysate (10 µg/Lane)
Lane 6: Rat brain tissue lysate (20 µg/Lane)
Lane 7: Mouse brain tissue lysate (20 µg/Lane)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 37 kDa
Observed band size: 25/35/40 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500422) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunocytochemistry analysis of SiHa cells labeling Cathepsin S with Rabbit anti-Cathepsin S antibody (HA500422) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cathepsin S antibody (HA500422) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution.
product information
SKU :
HA500422
Target name :
Cathepsin S
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IF-Cell
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human Cathepsin S aa 101-331 / 331.
Uniprot id :
P25774>SwissProt: P25774 Human;SwissProt: O70370 Mouse;SwissProt: Q02765 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
THP-1 cell lysate, Hela cell lysate, A431 cell lysate, PC-12 cell lysate, rat brain tissue lysate, mouse brain tissue lysate, SiHa.
Molecular wt :
Predicted band size: 37 kDa
Subcellular location :
Lysosome, Secreted, Cytoplasmic vesicle, phagosome.
Concentration :
2.305 mg/mL.
Recommended dilutions :
WB: 1:1,000;IF-Cell: 1:200
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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