KMT2D | HUABIO HA500417 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

KMT2D

catalog :

HA500417

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of KMT2D on recombinant protein with Rabbit anti-KMT2D antibody (HA500417) at 1/10,000 dilution. Lysates/proteins at 50 ng/Lane. Observed band size: 22 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500417) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of A549 cells labeling KMT2D with Rabbit anti-KMT2D antibody (HA500417) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KMT2D antibody (HA500417) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

product information

SKU :

HA500417

Target name :

KMT2D

Species reactivity :

Human

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within C terminal human KMT2D.

Uniprot id :

O14686>SwissProt: O14686 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

A549.

Molecular wt :

Predicted band size: 593 kDa

Subcellular location :

Nucleus

Concentration :

2.047 mg/ml

Recommended dilutions :

WB: 1:500-2,000 ;IF-Cell: 1:100

more info or order :

HUABIO product webpage