RYK | HUABIO HA500416 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

RYK

catalog :

HA500416

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of RYK on different lysates with Rabbit anti-RYK antibody (HA500416) at 1/500 dilution. Lane 1: SH-SY5Y cell lysate (10 µg/Lane) Lane 2: NIH/3T3 cell lysate (10 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Lane 4: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 68 kDa Observed band size: 52 kDa (C terminal fragment) Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500416) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of RYK on MCF-7 cell lysates with Rabbit anti-RYK antibody (HA500416) at 1/10,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 68 kDa Observed band size: 42 kDa (intracellular domain) Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500416) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of NIH/3T3 cells labeling RYK with Rabbit anti-RYK antibody (HA500416) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RYK antibody (HA500416) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

product information

SKU :

HA500416

Target name :

RYK

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human RYK aa 300-550.

Uniprot id :

P34925>SwissProt: P34925 Human;SwissProt: Q01887 Mouse;Entrez Gene: 140585 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

SH-SY5Y cell lysate, NIH/3T3 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, MCF-7 cell lysates, NIH/3T3, SH-SY5Y.

Molecular wt :

Predicted band size: 68 kDa

Subcellular location :

Cytoplasm, Membrane, Nucleus.

Concentration :

1.777 mg/ml

Recommended dilutions :

WB: 1:500-1:10,000 ;IF-Cell: 1:200 ;FC: 1:500-1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA500416_4.jpg

Pic legend4 :

Flow cytometric analysis of NIH/3T3 cells labeling RYK. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500416, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA500416_5.jpg

Pic legend5 :

Flow cytometric analysis of SH-SY5Y cells labeling RYK. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500416, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage