product summary
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company name :
HUABIO
product type :
antibody
product name :
IL23R
catalog :
HA500387
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of IL23R on different lysates with Rabbit anti-IL23R antibody (HA500387) at 1/500 dilution.
Lane 1: MCF-7 cell lysate
Lane 2: 293 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 72 kDa
Observed band size: 43 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500387) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunocytochemistry analysis of SH-SY5Y cells labeling IL23R with Rabbit anti-IL23R antibody (HA500387) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IL23R antibody (HA500387) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
product information
SKU :
HA500387
Target name :
IL23R
Species reactivity :
Human
Applications :
WB,IF-Cell
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human IL23R aa 440-490.
Uniprot id :
Q5VWK5>SwissProt: Q5VWK5 Human
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
MCF-7 cell lysate, 293 cell lysate, SH-SY5Y.
Molecular wt :
Predicted band size: 72 kDa
Subcellular location :
Cell membrane
Concentration :
1.88 mg/ml
Recommended dilutions :
WB: 1:500;IF-Cell: 1:200
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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