CD23 | HUABIO HA500386 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD23

catalog :

HA500386

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD23 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500386, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 2 :

Flow cytometric analysis of CD23 was done on Raji cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500386, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA500386

Target name :

CD23

Species reactivity :

Human

Applications :

IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human CD23 aa 250-321 / 321 (Extracellular).

Uniprot id :

P06734>SwissProt:P06734 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Human tonsil tissue, Raji.

Molecular wt :

Predicted band size: 36 kDa.

Subcellular location :

Secreted, cell membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-P:1:50-1:200 ;FC:1:50-1:100

more info or order :

HUABIO product webpage