EIF2S1 | HUABIO HA500385 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

EIF2S1

catalog :

HA500385

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of EIF2S1 on different lysates with Rabbit anti-EIF2S1 antibody (HA500385) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: A431 cell lysate Lane 3: Raji cell lysate Lane 4: Hela cell lysate Lane 5: PC-12 cell lysate Lane 6: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 21 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500385) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of EIF2S1 on different lysates with Rabbit anti-EIF2S1 antibody (HA500385) at 1/500 dilution. Lane 1: Mouse colon tissue lysate Lane 2: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500385) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-EIF2S1 antibody (HA500385) at 1/1,900 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500385) at 1/1,900 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA500385

Target name :

EIF2S1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,FC,IF-Cell,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human EIF2S1 aa 116-315/315.

Uniprot id :

P05198>SwissProt: P05198 Human;SwissProt: Q6ZWX6 Mouse;SwissProt: P68101 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

HepG2 cell lysate, A431 cell lysate, Raji cell lysate, Hela cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, mouse colon tissue lysate, rat spleen tissue lysate, human kidney tissue, Hela, A375, NCI-H441.

Molecular wt :

Predicted band size: 36 kDa

Subcellular location :

Cytoplasm, Stress granule, cytosol, Mitochondrion.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1,000 ;IHC-P: 1:1,900 ;FC: 1:500-1:1,000 ;IF-Cell: 1:100-1:200 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA500385_4.jpg

Pic legend4 :

Flow cytometric analysis of Hela cells labeling EIF2S1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500385, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA500385_5.jpg

Pic legend5 :

Immunocytochemistry analysis of A375 cells labeling EIF2S1 with Rabbit anti-EIF2S1 antibody (HA500385) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-EIF2S1 antibody (HA500385) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) was used as the secondary antibody at 1/1,000 dilution.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA500385_6.jpg

Pic legend6 :

Immunocytochemistry analysis of NCI-H441 cells labeling EIF2S1 with Rabbit anti-EIF2S1 antibody (HA500385) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-EIF2S1 antibody (HA500385) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA500385_7.jpg

Pic legend7 :

EIF2S1 was immunoprecipitated from 0.2 mg HepG2 cell lysate with HA500385 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA500385 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HepG2 cell lysate (input) Lane 2: HA500385 IP in HepG2 cell lysate Lane 3: Rabbit IgG instead of HA500385 in HepG2 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 2 seconds; ECL: K1801

more info or order :

HUABIO product webpage