PPP2R1B | HUABIO HA500383 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PPP2R1B

catalog :

HA500383

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PPP2R1B on different lysates with Rabbit anti-PPP2R1B antibody (HA500383) at 1/2,000 dilution. Lane 1: Jurkat cell lysate (10 µg/Lane) Lane 2: Hela cell lysate (10 µg/Lane) Lane 3: RAW264.7 cell lysate (10 µg/Lane) Lane 4: 293 cell lysate (10 µg/Lane) Lane 5: Rat brain liver tissue lysate (20 µg/Lane) Lane 6: Rat liver tissue lysate (20 µg/Lane) Lane 7: Mouse brain tissue lysate (20 µg/Lane) Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500383) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-PPP2R1B antibody (HA500383) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500383) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunocytochemistry analysis of SiHa cells labeling PPP2R1B with Rabbit anti-PPP2R1B antibody (HA500383) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PPP2R1B antibody (HA500383) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

product information

SKU :

HA500383

Target name :

PPP2R1B

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human PPP2R1B aa 100-350.

Uniprot id :

P30154>SwissProt: P30154 Human;SwissProt: Q7TNP2 Mouse;SwissProt: Q4QQT4 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Jurkat cell lysate, Hela cell lysate, RAW264.7 cell lysate, 293 cell lysate, rat brain liver tissue lysate, rat liver tissue lysate, mouse brain tissue lysate, human pancreas tissue, SiHa.

Molecular wt :

Predicted band size: 66 kDa

Subcellular location :

Secreted, Cytoplasm.

Concentration :

2.4 mg/ml

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:600 ;IF-Cell: 1:200

more info or order :

HUABIO product webpage