DAB2P | HUABIO HA500378 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

DAB2P

catalog :

HA500378

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-DAB2P antibody (HA500378) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500378) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 2 :

Immunocytochemistry analysis of SH-SY5Y cells labeling DAB2P with Rabbit anti-DAB2P antibody (HA500378) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-DAB2P antibody (HA500378) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

product information

SKU :

HA500378

Target name :

DAB2P

Species reactivity :

Human

Applications :

IHC-P,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human DAB2IP aa 950-1189.

Uniprot id :

Q5VWQ8>SwissProt: Q5VWQ8 Human;SwissProt: Q3UHC7 Mouse;SwissProt: Q6P730 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Mouse brain tissue lysate, rat brain tissue lysate, Hela cell lysate, A431 cell lysate, human thyroid carcinoma tissue, SH-SY5Y.

Molecular wt :

Predicted band size: 132 kDa

Subcellular location :

Cell membrane, Cytoplasm.

Concentration :

2.0 mg/ml

Recommended dilutions :

IHC-P: 1:800 ;IF-Cell: 1:200

more info or order :

HUABIO product webpage