RPS27A | HUABIO HA500371 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

RPS27A

catalog :

HA500371

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of RPS27A on different lysates with Rabbit anti-RPS27A antibody (HA500371) at 1/500 dilution. Lane 1: Hela cell lysate (10 µg/Lane) Lane 2: Jurkat cell lysate (10 µg/Lane) Lane 3: HepG2 cell lysate (10 µg/Lane) Lane 4: Rat liver tissue lysate (20 µg/Lane) Predicted band size: 18 kDa Observed band size: 15 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500371) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-RPS27A antibody (HA500371) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500371) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunocytochemistry analysis of A172 cells labeling RPS27A with Rabbit anti-RPS27A antibody (HA500371) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RPS27A antibody (HA500371) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

product information

SKU :

HA500371

Target name :

RPS27A

Species reactivity :

Human,Rat,Mouse

Applications :

WB,IHC-P,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human RPS27A aa 77-150.

Uniprot id :

P62979>SwissProt: P62979 Human;SwissProt: P62983 Mouse;SwissProt: P62982 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Hela cell lysate, Jurkat cell lysate, HepG2 cell lysate, rat liver tissue lysate, human kidney tissue, A172, A375.

Molecular wt :

Predicted band size: 18 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1.98 mg/ml

Recommended dilutions :

WB: 1:500;IHC-P: 1:600;IF-Cell: 1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA500371_4.jpg

Pic legend4 :

Immunocytochemistry analysis of A375 cells labeling RPS27A with Rabbit anti-RPS27A antibody (HA500371) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RPS27A antibody (HA500371) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

more info or order :

HUABIO product webpage