IFNGR2 | HUABIO HA500363 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

IFNGR2

catalog :

HA500363

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of IFNGR2 on different lysates with Rabbit anti-IFNGR2 antibody (HA500363) at 1/500 dilution. Lane 1: Jurkat cell lysate Lane 2: Hela cell lysate Lane 3: A431 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 38 kDa Observed band size: 40 kDa Exposure time: 1 minute; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500363) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of IFNGR2 on human skeletal muscle tissue lysates with Rabbit anti-IFNGR2 antibody (HA500363) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 38 kDa Observed band size: 40 kDa Exposure time: 1 minute; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500363) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of SH-SY5Y cells labeling IFNGR2 with Rabbit anti-IFNGR2 antibody (HA500363) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IFNGR2 antibody (HA500363) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

product information

SKU :

HA500363

Target name :

IFNGR2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human INGR2 22-71.

Uniprot id :

P38484>SwissProt: P38484 Human;Entrez Gene: 15980 Mouse;Entrez Gene: 360697 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Jurkat cell lysate, Hela cell lysate, A431 cell lysate, human skeletal muscle tissue lysates, SH-SY5Y.

Molecular wt :

Predicted band size: 38 kDa

Subcellular location :

Endoplasmic reticulum, Plasma membrane, Golgi apparatus.

Concentration :

1.8 mg/ml

Recommended dilutions :

WB: 1:500 ;IF-Cell: 1:200

more info or order :

HUABIO product webpage