USP8 | HUABIO HA500362 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

USP8

catalog :

HA500362

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of USP8 on different lysates with Rabbit anti-USP8 antibody (HA500362) at 1/500 dilution. Lane 1: A375 cell lysate (10 µg/Lane) Lane 2: A431 cell lysate (10 µg/Lane) Lane 3: HepG2 cell lysate (10 µg/Lane) Lane 4: 293T cell lysate (10 µg/Lane) Lane 5: MCF-7 cell lysate (10 µg/Lane) Lane 6: Mouse testis tissue lysate (20 µg/Lane) Lane 7: SK-Br-3 cell lysate (10 µg/Lane) Lane 8: F9 cell lysate (10 µg/Lane) Lane 9: Rat lung tissue lysate (20 µg/Lane) Lane 10: Hela cell lysate (10 µg/Lane) Predicted band size: 128 kDa Observed band size: 128 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500362) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-USP8 antibody (HA500362) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500362) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-USP8 antibody (HA500362) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500362) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA500362

Target name :

USP8

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human USP8 aa 1-250.

Uniprot id :

P40818>SwissProt: P40818 Human;SwissProt: Q80U87 Mouse;Entrez Gene: 296121 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

A375 cell lysate, A431 cell lysate, HepG2 cell lysate, 293T cell lysate, MCF-7 cell lysate, mouse testis tissue lysate, SK-Br-3 cell lysate, F9 cell lysate, rat lung tissue lysate, Hela cell lysate, mouse kidney tissue, human colon carcinoma tissue.

Molecular wt :

Predicted band size: 128 kDa

Subcellular location :

Cytoplasm, Cell membrane, Nucleus.

Concentration :

2.3 mg/ml

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:1,000

more info or order :

HUABIO product webpage