PSMC5 | HUABIO HA500352 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PSMC5

catalog :

HA500352

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PSMC5 on different lysates with Rabbit anti-PSMC5 antibody (HA500352) at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: Hela cell lysate Lane 3: 293T cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500352) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of PSMC5 on different lysates with Rabbit anti-PSMC5 antibody (HA500352) at 1/500 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500352) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PSMC5 antibody (HA500352) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500352) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA500352

Target name :

PSMC5

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human PSMC5 aa 205-406 / 406.

Uniprot id :

P62195>SwissProt: P62195 Human;SwissProt: P62196 Mouse;SwissProt: P62198 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

MCF-7 cell lysate, Hela cell lysate, 293T cell lysate, mouse heart tissue lysate, mouse brain tissue lysate, rat testis tissue lysate, rat brain tissue, human colon tissue, human skin tissue, human kidney tissue.

Molecular wt :

Predicted band size: 46 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IHC-P: 1:1,500

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA500352_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-PSMC5 antibody (HA500352) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500352) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA500352_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-PSMC5 antibody (HA500352) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500352) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA500352_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PSMC5 antibody (HA500352) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500352) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage