product summary
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company name :
HUABIO
product type :
antibody
product name :
YTHDF1
catalog :
HA500350
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of YTHDF1 on different lysates with Rabbit anti-YTHDF1 antibody (HA500350) at 1/1,000 dilution.
Lane 1: MCF-7 cell lysate
Lane 2: PC-12 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500350) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of YTHDF1 on NIH/3T3 cell lysates with Rabbit anti-YTHDF1 antibody (HA500350) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500350) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
image 3 :
Immunocytochemistry analysis of NIH/3T3 cells labeling YTHDF1 with Rabbit anti-YTHDF1 antibody (HA500350) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-YTHDF1 antibody (HA500350) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution.
product information
SKU :
HA500350
Target name :
YTHDF1
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IF-Cell,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Full length of Human YTHDF1.
Uniprot id :
Q9BYJ9>SwissProt: Q9BYJ9 Human;SwissProt: P59326 Mouse;Entrez Gene: 296467 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
MCF-7 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysates, NIH/3T3, rat brain tissue, mouse brain tissue.
Molecular wt :
Predicted band size: 61 kDa
Subcellular location :
Cytoplasm, P-body.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-1:1,000;IF-Cell: 1:100;IHC-P: 1:2,000
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA500350_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-YTHDF1 antibody (HA500350) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500350) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA500350_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-YTHDF1 antibody (HA500350) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500350) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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