TMED5 | HUABIO HA500347 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TMED5

catalog :

HA500347

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TMED5 on different lysates with Rabbit anti-TMED5 antibody (HA500347) at 1/100,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: RAW264.7 cell lysate (10 µg/Lane) Lane 3: C6 cell lysate (10 µg/Lane) Lane 4: Mouse liver tissue lysate (10 µg/Lane) Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500347) at 1/100,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded rat bladder tissue with Rabbit anti-TMED5 antibody (HA500347) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500347) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-TMED5 antibody (HA500347) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500347) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA500347

Target name :

TMED5

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human TMED5 aa 1-200 / 229.

Uniprot id :

Q9Y3A6>SwissProt: Q9Y3A6 Human;SwissProt: Q9CXE7 Mouse;SwissProt: Q6AXN3 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

HeLa cell lysate, RAW264.7 cell lysate, C6 cell lysate, Mouse liver tissue lysate, rat bladder tissue, mouse large intestine tissue.

Molecular wt :

Predicted band size: 26 kDa

Subcellular location :

Cis-Golgi network membrane, endoplasmic reticulum membrane, endoplasmic reticulum-Golgi intermediate compartment membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:100,000 ;IHC-P: 1:600-1:5,000

more info or order :

HUABIO product webpage