TNF Receptor II | HUABIO HA500332 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TNF Receptor II

catalog :

HA500332

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-TNF Receptor II antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500332, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 2 :

Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-TNF Receptor II antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500332, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TNF Receptor II antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500332, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA500332

Target name :

TNF Receptor II

Species reactivity :

Human,Mouse,Rat

Applications :

IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human TNFR2 aa 20-461 / 461.

Uniprot id :

P20333>SwissProt: P20333 Human;SwissProt: P25119 Mouse;SwissProt: Q80WY6 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Rat epididymis tissue, human uterus tissue, mouse testis tissue, HepG2.

Molecular wt :

48 kDa

Subcellular location :

Cell membrane, Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-P:1:50-1:100 ;FC:1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA500332_4.jpg

Pic legend4 :

Flow cytometric analysis of TNF Receptor II was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500332, 1/50) (yellow). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

more info or order :

HUABIO product webpage