product summary
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company name :
HUABIO
product type :
antibody
product name :
WSX-1
catalog :
HA500324
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, flow cytometry
more info or order :
image
image 1 :
Western blot analysis of WSX-1 on different lysates with Rabbit anti-WSX-1 antibody (HA500324) at 1/500 dilution.
Lane 1: A549 cell lysate
Lane 2: Hela cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 69 kDa
Observed band size: 74 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500324) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of WSX-1 on LNCAP cell lysates with Rabbit anti-WSX-1 antibody (HA500324) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: 30 seconds;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500324) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
image 3 :
Flow cytometric analysis of K562 cells labeling WSX-1.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA500324, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
product information
SKU :
HA500324
Target name :
WSX-1
Species reactivity :
Human
Applications :
WB,FC
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human WSX-1 aa 71-120.
Uniprot id :
Q6UWB1>SwissProt: Q6UWB1 Human
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
A549 cell lysate, Hela cell lysate, LNCAP cell lysates, K562.
Molecular wt :
Predicted band size: 69 kDa
Subcellular location :
Membrane
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500
;FC: 1:500-1:1,000
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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