product summary
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company name :
HUABIO
product type :
antibody
product name :
AE 1
catalog :
HA500323
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of AE 1 on different lysates with Rabbit anti-AE 1 antibody (HA500323) at 1/1,000 dilution.
Lane 1: K-562 cell lysate (20 µg/Lane)
Lane 2: Mouse kidney tissue lysate (40 µg/Lane)
Lane 3: Rat kidney tissue lysate (40 µg/Lane)
Lane 4: Mouse liver tissue lysate (40 µg/Lane)
Lane 5: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 102 kDa
Observed band size: 102 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500323) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-AE 1 antibody (HA500323) at 1/8,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500323) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-AE 1 antibody (HA500323) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500323) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA500323
Target name :
AE 1
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,FC
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human AE 1 aa 862-911.
Uniprot id :
P02730>SwissProt: P02730 Human;SwissProt: P04919 Mouse;SwissProt: P23562 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
K-562 cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, Mouse liver tissue lysate, Rat liver tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue, K562.
Molecular wt :
Predicted band size: 102 kDa
Subcellular location :
Cell membrane, Basolateral cell membrane.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:5,000
;IHC-P: 1:4,000-1:8,000
;FC: 1:500-1:1,000
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA500323_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-AE 1 antibody (HA500323) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500323) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA500323_5.jpg
Pic legend5 :
Flow cytometric analysis of K562 cells labeling AE 1.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA500323, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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