product summary
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company name :
HUABIO
product type :
antibody
product name :
IL-10RB
catalog :
HA500322
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, flow cytometry
more info or order :
image
image 1 :
Western blot analysis of IL-10RB on different lysates with Rabbit anti-IL-10RB antibody (HA500322) at 1/500 dilution.
Lane 1: 293T cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Hela cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 37 kDa
Observed band size: 42 kDa (Glycoprotein)
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500322) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunocytochemistry analysis of SiHa cells labeling IL-10RB with Rabbit anti-IL-10RB antibody (HA500322) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IL-10RB antibody (HA500322) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
image 3 :
Flow cytometric analysis of THP-1 cells labeling IL-10RB.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA500322, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
product information
SKU :
HA500322
Target name :
IL-10RB
Species reactivity :
Human
Applications :
WB,IF-Cell,FC
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human IL-10RB aa 170-220 (Extracellular domain).
Uniprot id :
Q08334>SwissProt: Q08334 Human
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
293T cell lysate, Jurkat cell lysate, Hela cell lysate, SiHa, THP-1.
Molecular wt :
Predicted band size: 37 kDa
Subcellular location :
Membrane
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500
;IF-Cell: 1:200
;FC: 1:500-1,000
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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