product summary
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company name :
HUABIO
product type :
antibody
product name :
ATP1B4
catalog :
HA500311
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of ATP1B4 on different lysates with Rabbit anti-ATP1B4 antibody (HA500311) at 1/2,000 dilution.
Lane 1: Mouse skeletal muscle tissue lysate
Lane 2: Rat skeletal muscle tissue lysate
Lane 3: Mouse heart tissue lysate (low expression)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500311) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of ATP1B4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500311, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human skeletal muscle tissue lysate
Lane 2: Mouse skeletal muscle tissue lysate
Lane 3: Mouse tongue tissue lysate
Lane 4: 293 cell lysate
product information
SKU :
HA500311
Target name :
ATP1B4
Species reactivity :
Human,Mouse,Rat
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human ATP1B4 100-300 / 357.
Uniprot id :
Q9UN42>SwissProt: Q9UN42 Human;SwissProt: Q99ME6 Mouse;SwissProt: Q9R193 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate, Mouse heart tissue lysate, Human skeletal muscle tissue lysate, Mouse tongue tissue lysate, 293 cell lysate.
Molecular wt :
Predicted band size: 42 kDa
Subcellular location :
Membrane, Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-1:2,000
Advanced Validation :
Relative expression (RE)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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