Adipose Triglyceride Lipase | HUABIO HA500301 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Adipose Triglyceride Lipase

catalog :

HA500301

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Adipose Triglyceride Lipase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500301, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: HepG2 cell lysate Lane 3: A431 cell lysate

image 2 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Adipose Triglyceride Lipase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500301, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA500301

Target name :

Adipose Triglyceride Lipase

Species reactivity :

Human

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Adipose Triglyceride Lipase aa 450-504 / 504 (Lumenal).

Uniprot id :

Q96AD5>SwissProt: Q96AD5 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

293 cell lysate, HepG2 cell lysate, A431 cell lysate, human pancreas tissue.

Molecular wt :

55 kDa

Subcellular location :

Cell membrane, Lipid droplet.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IHC-P: 1:100-1:500

more info or order :

HUABIO product webpage