product summary
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company name :
HUABIO
product type :
antibody
product name :
HLA E
catalog :
HA500299
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, flow cytometry
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA500299 image 1
Western blot analysis of HLA E on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500299, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell lysate Lane 2: THP-1 cell lysate Lane 3: HL-60 cell lysate
image 2 :
HUABIO HA500299 image 2
Flow cytometric analysis of HLA E was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500299, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
product information
SKU :
HA500299
Target name :
HLA E
Species reactivity :
Human
Applications :
WB,FC
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human HLA E aa 22-200 / 358 (Extracellular).
Uniprot id :
P13747>SwissProt: P13747 Human
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
Raji cell lysate, THP-1 cell lysate, HL-60 cell lysate, SiHa.
Molecular wt :
40 kDa
Subcellular location :
Golgi apparatus membrane, Cell membrane, Secreted.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-1:2,000 ;FC: 1:100-1:500
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!