product summary
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company name :
HUABIO
product type :
antibody
product name :
IL-21R
catalog :
HA500290
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of IL-21R on different lysates with rabbit anti-IL-21R antibody (HA500290) at 1/500 dilution.
Lane 1: Rat spleen tissue lysate
Lane 2: Rat liver tissue lysate
Lane 3: Rat thymus tissue lysate
Lysates/proteins at 10 µg per lane.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500290, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Predicted band size: 59 kDa
Observed band size: 80 kDa (Glycosylation)
image 2 :
Western blot analysis of IL-21R on different lysates with rabbit anti-IL-21R antibody (HA500290) at 1/500 dilution.
Lane 1: Mouse spleen tissue lysate
Lane 2: Mouse thymus tissue lysate
Lane 3: Daudi cell lysate
Lysates/proteins at 10 µg per lane.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500290, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Predicted band size: 59 kDa
Observed band size: 80 kDa (Glycosylation)
image 3 :
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-21R antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500290, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA500290
Target name :
IL-21R
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human IL-21R aa 51-100.
Uniprot id :
Q9HBE5>SwissProt: Q9HBE5 Human;SwissProt: Q9JHX3 Mouse;Entrez Gene: 308977 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
Rat spleen tissue lysate, rat liver tissue lysate, rat thymus tissue lysate, mouse spleen tissue lysate, mouse thymus tissue lysate, Daudi cell lysate, human spleen tissue.
Molecular wt :
Predicted band size: 59 kDa
Subcellular location :
Membrane
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500
;IHC-P: 1:600
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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