IFNAR2 | HUABIO HA500284 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

IFNAR2

catalog :

HA500284

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-IFNAR2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500284, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 2 :

ICC staining of IFNAR2 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500284, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

image 3 :

ICC staining of IFNAR2 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500284, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

HA500284

Target name :

IFNAR2

Species reactivity :

Human

Applications :

IHC-P,IF-Cell,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human IFNAR2 aa 194-243 (Extracellular domain).

Uniprot id :

P48551>SwissProt: P48551 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Human prostate tissue, SH-SY5Y, SiHa.

Molecular wt :

Predicted band size: 58 kDa

Subcellular location :

Cell membrane; Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-P: 1:600 ;IF-Cell: 1:200 ;IF-Tissue: 1:500

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA500284_4.jpg

Pic legend4 :

Application: IF-Tissue Species: Human Site: prostate Sample: Paraffin-embedded section Antibody concentration: 1/500

more info or order :

HUABIO product webpage