GCSF Receptor | HUABIO HA500282 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

GCSF Receptor

catalog :

HA500282

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of GCSF Receptor on different lysates with rabbit anti-GCSF Receptor antibody (HA500282) at 1/500 dilution. Lane 1: HL-60 cell lysate Lane 2: THP-1 cell lysate Lane 3: SH-SY5Y cell lysate Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500282, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 92 kDa Observed band size: 92 kDa

image 2 :

ICC staining of GCSF Receptor in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500282, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

image 3 :

Flow cytometric analysis of THP-1 cells labeling GCSF Receptor. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500282, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA500282

Target name :

GCSF Receptor

Species reactivity :

Human

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human GCSF Receptor aa 787-836.

Uniprot id :

Q99062>SwissProt: Q99062 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

HL-60 cell lysate, THP-1 cell lysate, SH-SY5Y cell lysate, Hela, THP-1.

Molecular wt :

Predicted band size: 92 kDa

Subcellular location :

Secreted, Cell membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;FC: 1:500-1:1,000

more info or order :

HUABIO product webpage