PLK1 | HUABIO HA500281 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PLK1

catalog :

HA500281

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PLK1 on different lysates with Rabbit anti-PLK1 antibody (HA500281) at 1/1,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa treated with 100nM Nocodazole for 24 hours whole cell lysate Lane 3: HeLa treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours whole cell lysate Lane 4: HT-29 whole cell lysate Lane 5: HT-29 treated with 100nM Nocodazole for 24 hours whole cell lysate Lane 6: HT-29 treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours whole cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500281) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of PLK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500281, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: Mouse testis tissue lysate Predicted band size: 68 kDa Observed band size: 68/55/40 kDa

image 3 :

Flow cytometric analysis of PLK1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500281, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA500281

Target name :

PLK1

Species reactivity :

Human,Mouse

Applications :

WB,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human PLK1 aa 1-50 / 603.

Uniprot id :

P53350>SwissProt: P53350 Human;SwissProt: Q07832 Mouse

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

HeLa whole cell lysate, HeLa treated with 100nM Nocodazole for 24 hours whole cell lysate, HeLa treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours whole cell lysate, HT-29 whole cell lysate, HT-29 treated with 100nM Nocodazole for 24 hours whole cell lysate, HT-29 treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours whole cell lysate, HepG2 cell lysate, Hela cell lysate, mouse testis tissue lysate, Hela.

Molecular wt :

Predicted band size: 68 kDa

Subcellular location :

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;FC: 1:500-1:1,000

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage