Peptide YY | HUABIO HA500250 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Peptide YY

catalog :

HA500250

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Peptide YY on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500250, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human small intestine tissue lysate Lane 2: RAW264.7 cell lysate Lane 1: Rat liver tissue lysate Predicted band size: 11 kDa. Observed band size: 20 kDa.

image 2 :

ICC staining of Peptide YY in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500250, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

image 3 :

Flow cytometric analysis of Peptide YY was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500250, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA500250

Target name :

Peptide YY

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within N-terminal human Peptide YY.

Uniprot id :

P10082>SwissProt: P10082 Human;SwissProt: Q9EPS2 Mouse;SwissProt: P10631 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Human small intestine tissue lysate, RAW264.7 cell lysate, rat liver tissue lysate, SW620, SiHa.

Molecular wt :

Predicted band size: 11 kDa.

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:200 ;FC: 1:100-1:500

more info or order :

HUABIO product webpage