SF3A1 | HUABIO HA500235 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

SF3A1

catalog :

HA500235

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of SF3A1 on different lysates with Rabbit anti-SF3A1 antibody (HA500235) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (15 µg/Lane) Lane 2: HeLa cell lysate (15 µg/Lane) Lane 3: A549 cell lysate (15 µg/Lane) Lane 4: K-562 cell lysate (15 µg/Lane) Lane 5: Mouse brain tissue lysate (30 µg/Lane) Lane 6: Rat lung tissue lysate (30 µg/Lane) Predicted band size: 89 kDa Observed band size: 110 kDa Exposure time: 2 minutes 48 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500235) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-SF3A1 antibody (HA500235) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500235) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-SF3A1 antibody (HA500235) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500235) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA500235

Target name :

SF3A1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human SF3A1 aa 50-100 / 793.

Uniprot id :

Q15459>SwissProt: Q15459 Human;SwissProt: Q8K4Z5 Mouse;Entrez Gene: 305479 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Jurkat cell lysate, HeLa cell lysate, A549 cell lysate, K-562 cell lysate, mouse brain tissue lysate, rat lung tissue lysate, human colon carcinoma tissue, mouse brain tissue, mouse lung tissue.

Molecular wt :

Predicted band size: 89 kDa

Subcellular location :

Nucleus, Nucleus speckle.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IHC-P: 1:50-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA500235_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-SF3A1 antibody (HA500235) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500235) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage