UBE2D3 | HUABIO HA500230 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

UBE2D3

catalog :

HA500230

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of UBE2D3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500230, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: 293T cell lysate Lane 3: MCF-7 cell lysate

image 2 :

Western blot analysis of UBE2D3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500230, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat liver tissue lysate Lane 2: Mouse lung tissue lysate

image 3 :

ICC staining of UBE2D3 in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500230, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

HA500230

Target name :

UBE2D3

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant full length protein of human UBE2D3.

Uniprot id :

P61077>SwissProt: P61077 Human;SwissProt: P61079 Mouse;SwissProt: P61078 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Jurkat cell lysate, 293T cell lysate, MCF-7 cell lysate, rat liver tissue lysate, mouse lung tissue lysate, SW620, mouse brain tissue.

Molecular wt :

Predicted band size: 17 kDa.

Subcellular location :

Cell membrane, Endosome membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IF-Cell: 1:50-1:100 ;IHC-P: 1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA500230_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-UBE2D3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500230, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage