GOLPH3L | HUABIO HA500205 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

GOLPH3L

catalog :

HA500205

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of GOLPH3L on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500205, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Daudi cell lysate Lane 3: MCF-7 cell lysate Lane 4: SK-Br-3 cell lysate Lane 5: Rat ovary tissue lysate

image 2 :

Western blot analysis of GOLPH3L on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500205, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat large intestine tissue lysate Lane 2: Mouse colon tissue lysate

image 3 :

ICC staining of GOLPH3L in A549 cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500205, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

HA500205

Target name :

GOLPH3L

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human GOLPH3L aa 50-250.

Uniprot id :

Q9H4A5>SwissProt: Q9H4A5 Human;SwissProt: Q8R088 Mouse;SwissProt: Q66H74 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Hela cell lysate, Daudi cell lysate, MCF-7 cell lysate, SK-Br-3 cell lysate, rat ovary tissue lysate, rat large intestine tissue lysate, mouse colon tissue lysate, A549, Hela.

Molecular wt :

Predicted band size: 33 kDa

Subcellular location :

Golgi stack membrane, trans-Golgi network membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:100 ;FC: 1:500-1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA500205_4.jpg

Pic legend4 :

Flow cytometric analysis of GOLPH3L was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500205, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage