Wnt16 | HUABIO HA500145 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Wnt16

catalog :

HA500145

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Wnt16 on Raji cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500145, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Wnt16 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500145, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse spleen tissue lysate Lane 2: Rat kidney tissue lysate

image 3 :

ICC staining of Wnt16 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500145, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

HA500145

Target name :

Wnt16

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Wnt16 aa 20-60.

Uniprot id :

Q9UBV4>SwissProt: Q9UBV4 Human;SwissProt: Q9QYS1 Mouse

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Raji cell lysates, mouse spleen tissue lysate, rat kidney tissue lysate, 293T, mouse kidney tissue.

Molecular wt :

41 kDa

Subcellular location :

Extracellular matrix

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000;IF-Cell: 1:50-1:200;IHC-P: 1:500-1:2,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA500145_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Wnt16 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500145, 1/1,500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage