OC-3/2/1 | HUABIO HA500130 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

OC-3/2/1

catalog :

HA500130

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of OC-3/2/1 on different lysates with Rabbit anti-OC-3/2/1 antibody (HA500130) at 1/2,000 dilution. Lane 1: SW480 cell lysate Lane 2: HeLa cell lysate Lane 3: AGS cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500130) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Flow cytometric analysis of OC-3/2/1 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500130, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA500130

Target name :

OC-3/2/1

Species reactivity :

Human

Applications :

WB,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human ONECUT3 aa 430-470.

Uniprot id :

O60422>SwissProt: O60422 Human;SwissProt: O95948 Human;SwissProt: Q9UBC0 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

SW480 cell lysate, HeLa cell lysate, AGS cell lysate, SW620.

Molecular wt :

Predicted band size: 50 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;FC: 1:50-1:100

more info or order :

HUABIO product webpage