Phospho-AKT2 (S474) | HUABIO HA500116 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-AKT2 (S474)

catalog :

HA500116

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-AKT2 (S474) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500116, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Untreated Hela whole cell lysates Lane 2: Hela treated with Calyculin A whole cell lysates Lane 3: Hela treated with insulin whole cell lysates

image 2 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-AKT2 (S474) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500116, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Phospho-AKT2 (S474) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500116, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA500116

Target name :

Phospho-AKT2 (S474)

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human AKT2 aa 450-481(phospho S474).

Uniprot id :

P31751>SwissProt: P31751 Human;SwissProt: Q60823 Mouse

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Hela treated with Calyculin A whole cell lysate, Hela treated with insulin whole cell lysate, human kidney tissue, mouse testis tissue.

Molecular wt :

56 kDa

Subcellular location :

Cell membrane, Nucleus, Early endosome, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IHC-P: 1:100-1:500

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage