p70 S6 Kinase | HUABIO HA500095 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

p70 S6 Kinase

catalog :

HA500095

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of p70 S6 Kinase on different lysates with Rabbit anti-p70 S6 Kinase antibody (HA500095) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: C6 cell lysate Lane 3: PC-12 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: Mouse thymus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500095) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-p70 S6 Kinase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500095, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA500095

Target name :

p70 S6 Kinase

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human S6K1 aa 346-525.

Uniprot id :

P23443>SwissProt: P23443 Human;SwissProt: Q8BSK8 Mouse;SwissProt: P67999 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

HeLa cell lysate, C6 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, mouse thymus tissue lysate, mouse testis tissue.

Molecular wt :

Predicted band size: 59 kDa.

Subcellular location :

Mitochondrion outer membrane, Mitochondrion, Nucleus, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IHC-P: 1:100-1:500

more info or order :

HUABIO product webpage