DDX3 | HUABIO HA500093 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

DDX3

catalog :

HA500093

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of DDX3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500093, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate Lane 2: K562 cell lysate Lane 3: A431 cell lysate Lane 4: HepG2 cell lysate Lane 5: Jurkat cell lysate Lane 6: Rat testis tissue lysate

image 2 :

ICC staining of DDX3 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500093, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

image 3 :

ICC staining of DDX3 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500093, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

HA500093

Target name :

DDX3

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human DDX3 aa 220-420.

Uniprot id :

O00571>SwissProt: O00571 Human;SwissProt: Q62167 Mouse

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

NIH/3T3 cell lysate, K562 cell lysate, A431 cell lysate, HepG2 cell lysate, Jurkat cell lysate, rat testis tissue lysate, A549, LOVO.

Molecular wt :

73 kDa

Subcellular location :

Inflammasome, Centrosome, Cell membrane, Nucleus, Cytoplasm, Stress granule, Lamellipodium.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:100

more info or order :

HUABIO product webpage