GABRB3 | HUABIO HA500090 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

GABRB3

catalog :

HA500090

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of GABRB3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500090, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat brain tissue lysate Lane 2: Rat cerebellum tissue lysate Lane 3: Mouse brain tissue lysate Lane 4: Mouse cerebellum tissue lysate Lane 5: HepG2 cell lysate Lane 6: 293 cell lysate

image 2 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GABRB3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500090, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-GABRB3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500090, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA500090

Target name :

GABRB3

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human GABRB3 aa 400-443 (Cytoplasmic domain).

Uniprot id :

P28472>SwissProt: P28472 Human;SwissProt: P63080 Mouse;SwissProt: P63079 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Rat brain tissue lysate, rat cerebellum tissue lysate, mouse brain tissue lysate, mouse cerebellum tissue lysate, HepG2 cell lysate, 293 cell lysate, rat brain tissue, human lung tissue, mouse brain tissue.

Molecular wt :

Predicted band size: 54 kDa.

Subcellular location :

Plasma membrane, Cell membrane, Cytoplasmic vesicle membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IHC-P: 1:100-1:500

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA500090_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GABRB3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500090, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage