Ly6c | HUABIO HA500088 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Ly6c

catalog :

HA500088

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Ly6c on different lysates with Rabbit anti-Ly6c antibody (HA500088) at 1/1,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Mouse spleen tissue lysate Lane 3: Mouse testis tissue lysate (low expression) Lane 4: Mouse liver tissue lysate (low expression) Lane 5: Mouse kidney tissue lysate Lane 6: Mouse heart tissue lysate Lane 7: Mouse brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500088) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Ly6c antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500088, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Ly6c antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500088, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA500088

Target name :

Ly6c

Species reactivity :

Mouse

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within mouse Ly6c1 aa 75-100.

Uniprot id :

P0CW02>SwissProt: P0CW02 Mouse;SwissProt: P0CW03 Mouse

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Mouse lung tissue lysate, mouse spleen tissue lysate, mouse testis tissue lysate, mouse kidney tissue lysate, mouse heart tissue lysate, mouse brain tissue lysate, mouse lung tissue, mouse brain tissue, mouse spleen tissue, mouse spleen cells.

Molecular wt :

Predicted band size: 14 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IHC-P: 1:100-1:500 ;FC: 1:1,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA500088_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Ly6c antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500088, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA500088_5.jpg

Pic legend5 :

Flow cytometric analysis of mouse spleen cells labeling Ly6c (HA500088) and CD8-PE. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA500088, 1/1,000). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Compared with Rabbit IgG Isotype Control (iFluor™ 488, 1/1,000).

more info or order :

HUABIO product webpage