Scramblase 1 | HUABIO HA500063 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Scramblase 1

catalog :

HA500063

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunoprecipitation, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Scramblase 1 on different lysates with Rabbit anti-Scramblase 1 antibody (HA500063) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: HeLa cell lysate Lane 3: THP-1 cell lysate Lane 4: MDA-MB-231 cell lysate Lane 5: SH-SY5Y cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500063) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining of Scramblase 1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500063, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

image 3 :

Flow cytometric analysis of SK-MEL-28 cells labeling Scramblase 1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500063, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA500063

Target name :

Scramblase 1

Species reactivity :

Human

Applications :

WB,IF-Cell,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within N-terminal human Scramblase 1.

Uniprot id :

O15162>SwissProt: O15162 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

SK-MEL-28 cell lysate, HeLa cell lysate, THP-1 cell lysate, MDA-MB-231 cell lysate, HUVEC, SK-MEL-28.

Molecular wt :

Predicted band size: 35 kDa.

Subcellular location :

Cell membrane, Nucleus, Membrane, Cytoplasm, perinuclear region.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50-1:100 ;FC: 1:1,000 ;IP: 1-2μg/sample

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA500063_4.jpg

Pic legend4 :

Scramblase 1 was immunoprecipitated from 0.2 mg SK-MEL-28 cell lysate with HA500063 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA500063 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SK-MEL-28 cell lysate (input) Lane 2: HA500063 IP in SK-MEL-28 cell lysate Lane 3: Rabbit IgG instead of HA500063 in SK-MEL-28 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 14 seconds; ECL: K1801

more info or order :

HUABIO product webpage