product summary
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company name :
HUABIO
product type :
antibody
product name :
mCherry / TdTomato
catalog :
HA500049
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
application :
western blot, ELISA
more info or order :
image
image 1 :
Western blot analysis of mCherry / TdTomato on different lysates with Rabbit anti-mCherry / TdTomato antibody (HA500049) at 1/5,000 dilution.
Lane 1: 293T transfected with empty control cell lysate
Lane 2: 293T transfected with mCherry / TdTomato cell lysate
Lysates/proteins at 5 µg/Lane.
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500049) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of mCherry / TdTomato on tdTomato recombinant protein with Rabbit anti-mCherry / TdTomato antibody (HA500049) at 1/80,000 dilution.
Lysates/proteins at 50 ng/Lane.
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500049) at 1/80,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
ELISA analysis of with anti-mCherry antibody. Antigen (1 μg/mL). The antigen was used as the coating antigen, and the anti-mCherry antibody was used as the capture antigen for ELISA.
product information
SKU :
HA500049
Target name :
mCherry / TdTomato
Species reactivity :
Species independent
Applications :
WB,ELISA,IF-Cell
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within mCherry 1-236aa.
Uniprot id :
X5DSL3>SwissProt: X5DSL3 AnaplasmaMarginale
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
Recombinant mCherry protein lysates.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:5,000-1:80,000
;ELISA: 1:1,000-1:5,000
;IF-Cell: 1:10,000
Advanced Validation :
Cell treatment (CT)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA500049_4.jpg
Pic legend4 :
Immunocytochemistry analysis of HeLa cells transfected with or without mCherry labeling mCherry / TdTomato with Rabbit anti-mCherry / TdTomato antibody (HA500049) at 1/10,000 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-mCherry / TdTomato antibody (HA500049) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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