product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
OAS3
catalog :
HA500039
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of OAS3 on different lysates with Rabbit anti-OAS3 antibody (HA500039) at 1/1,000 dilution.
Lane 1: Mouse testis tissue lysate
Lane 2: Rat testis tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 126 kDa
Observed band size: 126 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500039) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of OAS3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500039, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A549 cell lysate
Lane 2: Human placental tissue lysate
Lane 3: A431 cell lysate
image 3 :
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-OAS3 antibody (HA500039) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500039) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA500039
Target name :
OAS3
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,IF-Cell
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human OAS3 aa 900-1059.
Uniprot id :
Q9Y6K5>SwissProt: Q9Y6K5 Human;SwissProt: Q8VI93 Mouse;SwissProt: Q5MYT7 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
Mouse testis tissue lysate, Rat testis tissue lysate, A549 cell lysate, Human placental tissue lysate, A431 cell lysate, human testis tissue, A594, LOVO.
Molecular wt :
Predicted band size: 121 kDa
Subcellular location :
Cytoplasm, Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-1:1,000
;IHC-P: 1:1,000
;IF-Cell: 1:50-1:200
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA500039_4.jpg
Pic legend4 :
Immunocytochemistry analysis of A594 cells labeling OAS3 with Rabbit anti-OAS3 antibody (HA500039) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-OAS3 antibody (HA500039) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA500039_5.jpg
Pic legend5 :
Immunocytochemistry analysis of LOVO cells labeling OAS3 with Rabbit anti-OAS3 antibody (HA500039) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-OAS3 antibody (HA500039) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
browse more products
questions and comments