product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
Desmocollin 3
catalog :
ET7111-26
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE55-86
reactivity :
human, mouse, rat
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of Desmocollin 3 on different lysates with Rabbit anti-Desmocollin 3 antibody (ET7111-26) at 1/2,000 dilution.
Lane 1: A431 cell lysate (RIPA lysis)
Lane 2: HCT 116 cell lysate (low expression) (RIPA lysis)
Lane 3: Mouse skin tissue lysate (RIPA lysis)
Lane 4: Mouse skin tissue lysate (hot lysis)
Lane 5: Rat skin tissue lysate (hot lysis)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-26) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Desmocollin 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-26, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded mouse esophagus tissue using anti-Desmocollin 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-26, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
ET7111-26
Target name :
Desmocollin 3
Species reactivity :
Human,Mouse,Rat
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within C-terminal Human Desmocollin 3.
Uniprot id :
Q14574>SwissProt: Q14574 Human;SwissProt: P55850 Mouse;Entrez Gene: 307563 Rat
Host :
Rabbit
Clone number :
JE55-86
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
A431 cell lysate, HCT 116 cell lysate, mouse skin tissue lysate, rat skin tissue lysate, human skin tissue, mouse esophagus tissue.
Molecular wt :
Predicted band size: 100 kDa.
Subcellular location :
Cell membrane, desmosome.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000
Advanced Validation :
Relative expression (RE)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
browse more products
questions and comments