CD35 | HUABIO ET7111-05 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD35

catalog :

ET7111-05

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE54-31

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD35 antibody (ET7111-05) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-05) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 2 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD35 antibody (ET7111-05) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-05) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CD35 antibody (ET7111-05) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-05) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET7111-05

Target name :

CD35

Species reactivity :

Human

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human CD35 aa 280-372 / 2,039.

Uniprot id :

P17927>SwissProt: P17927 Human

Host :

Rabbit

Clone number :

JE54-31

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human tonsil tissue, human spleen tissue, human kidney tissue.

Molecular wt :

Predicted band size: 224 kDa.

Subcellular location :

Membrane

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-P:1:50-1:200 ;WB:1:500

more info or order :

HUABIO product webpage