RanBP9 | HUABIO ET7110-95 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

RanBP9

catalog :

ET7110-95

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE55-10

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of RanBP9 on different lysates with Rabbit anti-RanBP9 antibody (ET7110-95) at 1/500 dilution. Lane 1: Mouse hippocampus tissue lysate (20 µg/Lane) Lane 2: Rat kidney tissue lysate (20 µg/Lane) Lane 3: SK-Br-3 cell lysate (10 µg/Lane) Predicted band size: 116 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-95) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-RanBP9 antibody (ET7110-95) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-95) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-RanBP9 antibody (ET7110-95) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-95) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET7110-95

Target name :

RanBP9

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human RANBP9 aa 90-205 / 1,041.

Uniprot id :

Q96P70>SwissProt: Q96P70 Human;SwissProt: Q91YE6 Mouse;Entrez Gene: 304817 Rat

Host :

Rabbit

Clone number :

JE55-10

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse hippocampus tissue lysate, rat kidney tissue lysate, SK-Br-3 cell lysate, rat testis tissue, human kidney tissue, mouse heart tissue.

Molecular wt :

Predicted band size: 116 kDa.

Subcellular location :

Nucleus, cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB:1:500-1:1,000 ;IHC-P:1:50-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7110-95_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-RanBP9 antibody (ET7110-95) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-95) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage