product summary
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company name :
HUABIO
product type :
antibody
product name :
Kallikrein 5
catalog :
ET7110-90
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE54-94
reactivity :
human
application :
western blot, flow cytometry
more info or order :
image
image 1 :
Western blot analysis of Kallikrein 5 on different lysates with Rabbit anti-Kallikrein 5 antibody (ET7110-90) at 1/500 dilution.
Lane 1: MCF-7 cell lysate
Lane 2: SK-Br-3 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 32 kDa
Observed band size: 40 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-90) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunocytochemistry analysis of MCF-7 cells labeling Kallikrein 5 with Rabbit anti-Kallikrein 5 antibody (ET7110-90) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Kallikrein 5 antibody (ET7110-90) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
image 3 :
Flow cytometric analysis of Kallikrein 5 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-90, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
product information
SKU :
ET7110-90
Target name :
Kallikrein 5
Species reactivity :
Human
Applications :
WB,IF-Cell,FC
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human Kallikrein 5 aa 161-293 / 293.
Uniprot id :
Q9Y337>SwissProt: Q9Y337 Human
Host :
Rabbit
Clone number :
JE54-94
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
MCF-7 cell lysate, SK-Br-3 cell lysate, MCF-7, A431.
Molecular wt :
Predicted band size: 32 kDa.
Subcellular location :
Secreted.
Concentration :
1 mg/mL.
Recommended dilutions :
WB:1:500-1:1,000
;IF-Cell:1:50-1:100
;FC:1:50-1:100
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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