TNFRSF19 | HUABIO ET7110-74 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TNFRSF19

catalog :

ET7110-74

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE54-51

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TNFRSF19 on different lysates with Rabbit anti-TNFRSF19 antibody (ET7110-74) at 1/500 dilution. Lane 1: mouse testis tissue lysate(20 µg/Lane) Lane 2: rat large intestine tissue lysate (20 µg/Lane) Lane 3: A431 cell lysate (10 µg/Lane) Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 1 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-74) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/5,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-TNFRSF19 antibody (ET7110-74) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-74) at 1/200 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-TNFRSF19 antibody (ET7110-74) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-74) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET7110-74

Target name :

TNFRSF19

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human TNFRSF19 aa 80-220 / 423.

Uniprot id :

Q9NS68>SwissProt: Q9NS68 Human;SwissProt: Q9JLL3 Mouse

Host :

Rabbit

Clone number :

JE54-51

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse testis tissue lysate, rat large intestine tissue lysate, A431 cell lysate, human prostate carcinoma tissue, human small intestine tissue, HepG2.

Molecular wt :

Predicted band size: 46 kDa

Subcellular location :

Membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB:1:500-1:1,000 ;IHC-P:1:50-1:200 ;FC:1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7110-74_4.jpg

Pic legend4 :

Flow cytometric analysis of TNFRSF19 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-74, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage