Estrogen Receptor alpha | HUABIO ET7110-60 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Estrogen Receptor alpha

catalog :

ET7110-60

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE26-14

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Estrogen Receptor alpha on different lysates with Rabbit anti-Estrogen Receptor alpha antibody (ET7110-60) at 1/5,000 dilution. Lane 1: MCF7 cell lysate (20 µg/Lane) Lane 2: T-47D cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (negative) (20 µg/Lane) Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-60) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-Estrogen Receptor alpha antibody (ET7110-60) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-60) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse endometrium tissue with Rabbit anti-Estrogen Receptor alpha antibody (ET7110-60) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-60) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET7110-60

Target name :

Estrogen Receptor alpha

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Estrogen Receptor alpha aa 1-50 / 595.

Uniprot id :

P03372>SwissProt: P03372 Human;SwissProt: P19785 Mouse

Host :

Rabbit

Clone number :

JE26-14

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MCF7 cell lysate, T-47D cell lysate, human endometrium tissue, mouse endometrium tissue.

Molecular wt :

Predicted band size: 66 kDa

Subcellular location :

Cell membrane, nucleus, cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB:1:2,000-1:5,000 ;IHC-P: 1:200

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage