MSH2 | HUABIO ET7110-50 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MSH2

catalog :

ET7110-50

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE28-48

reactivity :

human

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

All lanes: Western blot analysis of MSH2 with anti-MSH2 antibody [JE28-48] (ET7110-50) at 1/1,000 dilution. Lane 1: Wild-type SCC7 whole cell lysate (20 µg). Lane 2: MSH2 knockout SCC7 whole cell lysate (20 µg). ET7110-50 was shown to specifically react with MSH2 in wild-type SCC7 cells. No band was observed when MSH2 knockout sample was tested. Wild-type and MSH2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET7110-50, 1/1,000) and Loading control antibody(Rabbit anti-HSP90, ET1605-56, 1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of MSH2 on HL-60 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-50, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-MSH2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-50, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET7110-50

Target name :

MSH2

Species reactivity :

Human

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human MSH2 aa 1-100 (N terminal).

Uniprot id :

P43246>SwissProt: P43246 Human

Host :

Rabbit

Clone number :

JE28-48

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HL-60 cell lysates, Wild-type SCC7 whole cell lysates, human colon carcinoma tissue, Hela.

Molecular wt :

Predicted band size: 105 kDa

Subcellular location :

Nucleus, chromosome.

Concentration :

1 mg/mL

Recommended dilutions :

WB:1:500-1:2,000 ;IHC-P:1:50-1:200 ;FC:1:50-1:100

Advanced Validation :

Knockout (KO)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7110-50_4.jpg

Pic legend4 :

Flow cytometric analysis of MSH2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-50, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage