product summary
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company name :
HUABIO
product type :
antibody
product name :
NEK6
catalog :
ET7110-15
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE51-72
reactivity :
human
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of NEK6 on THP-1 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
image 2 :
All lanes: Western blot analysis of NEK6 with anti-NEK6 antibody[JE51-72] (ET7110-15) at 1/1,000 dilution.
Lane 1: Wild-type 293T whole cell lysate (30 µg).
Lane 2: NEK6 knockout 293T whole cell lysate (30 µg).
ET7110-15 was shown to specifically react with NEK6 in wild-type 293T cells. No band was observed when NEK6 knockout sample was tested. Wild-type and NEK6 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET7110-15, 1/1,000) was used in 5% BSA at room temperature for 2 hours.
product information
SKU :
ET7110-15
Target name :
NEK6
Species reactivity :
Human
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human NEK6 aa 170-313 / 313.
Uniprot id :
Q9HC98>SwissProt: Q9HC98 Human
Host :
Rabbit
Clone number :
JE51-72
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
THP-1 cell lysate, Wild-type 293T whole cell lysate.
Molecular wt :
Predicted band size: 36 kDa
Subcellular location :
Cytoplasm, Cytoskeleton, Microtubule, Nucleus.
Concentration :
1 mg/mL
Recommended dilutions :
WB: 1:500-1:2,000
Advanced Validation :
Knockout (KO)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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