EHMT2 / G9A | HUABIO ET7110-08 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

EHMT2 / G9A

catalog :

ET7110-08

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE50-84

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of EHMT2 / G9A on different lysates with Rabbit anti-EHMT2 / G9A antibody (ET7110-08) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-EHMT2 / G9A KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 132 kDa Observed band size: 160, 170 kDa Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-08) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-EHMT2 / G9A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-08, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-EHMT2 / G9A antibody (ET7110-08) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-08) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET7110-08

Target name :

EHMT2 / G9A

Species reactivity :

Human

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human G9A aa 1,060-1,210 / 1,210.

Uniprot id :

Q96KQ7>SwissProt: Q96KQ7 Human

Host :

Rabbit

Clone number :

JE50-84

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human colon carcinoma tissue, human breast carcinoma tissue, human stomach carcinoma tissue.

Molecular wt :

Predicted band size: 132 kDa.

Subcellular location :

Nucleus, chromosome.

Concentration :

1 mg/mL

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:50-1:400

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7110-08_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-EHMT2 / G9A antibody (ET7110-08) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-08) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage