NRG3 | HUABIO ET7109-98 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NRG3

catalog :

ET7109-98

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE49-84

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of NRG3 on different lysates with Rabbit anti-NRG3 antibody (ET7109-98) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 µg/Lane) Lane 2: C6 cell lysate (20 µg/Lane) Lane 3: Mouse brain tissue lysate (40 µg/Lane) Predicted band size: 78 kDa Observed band size: 50 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-98) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-NRG3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-98, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NRG3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-98, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET7109-98

Target name :

NRG3

Species reactivity :

Human,Rat,Mouse

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human NRG3 aa 560-720 / 720.

Uniprot id :

P56975>SwissProt: P56975 Human;SwissProt: O35181 Mouse;Entrez Gene: 498596 Rat

Host :

Rabbit

Clone number :

JE49-84

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SH-SY5Y cell lysate, C6 cell lysate, mouse brain tissue lysate, rat testis tissue, mouse brain tissue, F9.

Molecular wt :

Predicted band size: 78 kDa

Subcellular location :

Membrane. Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7109-98_4.jpg

Pic legend4 :

Flow cytometric analysis of NRG3 was done on F9 cells. The cells were fixed, permeabilized and stained with Carcino Embryonic Antigen CEA antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.

more info or order :

HUABIO product webpage