Lactoferrin | HUABIO ET7109-95 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Lactoferrin

catalog :

ET7109-95

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE49-60

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Lactoferrin on human placenta tissue lysates with Rabbit anti-Lactoferrin antibody (ET7109-95) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 78 kDa Observed band size: 78 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-95) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Lactoferrin on human kidney tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Lactoferrin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-95, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET7109-95

Target name :

Lactoferrin

Species reactivity :

Human

Applications :

WB,IF-Cell,IF-Tissue,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Lactoferrin aa 26-221 / 710.

Uniprot id :

P02788>SwissProt: P02788 Human

Host :

Rabbit

Clone number :

JE49-60

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human placenta tissue lysates, human kidney tissue lysates, human spleen tissue, human prostate cancer tissue.

Molecular wt :

Predicted band size: 78 kDa

Subcellular location :

Cytoplasm. Nucleus. Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB:1:500-1:2,000 ;IF-Cell:1:100 ;IF-Tissue:1:100 ;IF-Tissue:1:50-1:100 ;IHC-P:1:50-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7109-95_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Lactoferrin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-95, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage